منابع مشابه
Inactivation of Bacterial D-Amino Acid Transaminase by fi-Chloro-D-alanine*
Purified D-amino acid transaminase from Bacillus sphaericus catalyzes an (~,/3 elimination from the D isomer of pchloroalanine to yield equivalent amounts of pyruvate, chloride, and ammonia; the L isomer of chloroalanine is not a substrate for this transaminase. During the /I elimination there is a synchronous loss in enzyme activity; the Kinact for /I-chloroalanine was estimated to be about 10...
متن کاملInactivation of bacterial D-amino acid transaminase by beta-chloro-D-alanine.
Purified D-amino acid transaminase from Bacillus sphaericus catalyzes an alpha,beta elimination from the D isomer of beta-chloroalanine to yield equivalent amounts of pyruvate, chloride, and ammonia; the L isomer of chloroalanine is not a substrate for this transaminase. During the beta elimination there is a synchronous loss in enzyme activity; the Kinact for beta-chloroalanine was estimated t...
متن کاملMycobacterium smegmatis D-Alanine Racemase Mutants Are Not Dependent on D-Alanine for Growth.
Mycobacterium smegmatis is a fast-growing nonpathogenic species particularly useful in studying basic cellular processes of relevance to pathogenic mycobacteria. This study focused on the D-alanine racemase gene (alrA), which is involved in the synthesis of D-alanine, a basic component of peptidoglycan that forms the backbone of the cell wall. M. smegmatis alrA null mutants were generated by ho...
متن کاملMetabolomics analysis identifies d-Alanine-d-Alanine ligase as the primary lethal target of d-Cycloserine in mycobacteria.
d-Cycloserine is an effective second line antibiotic used as a last resort to treat multi (MDR)- and extensively (XDR) drug resistant strains of Mycobacterium tuberculosis . d-Cycloserine interferes with the formation of peptidoglycan biosynthesis by competitive inhibition of alanine racemase (Alr) and d-alanine-d-alanine ligase (Ddl). Although the two enzymes are known to be inhibited, the in ...
متن کاملComplexation of acetyl-D-alanyl-D-alanine by antibiotic A35512B.
Antibiotic A3 5512B1.2) is a member of the glycopeptide family of antibiotics; complete structures have been reported for two members of this group, vancomycin3' and ristocetin A4' a) . Both vancomycin and ristocetin have been shown to form complexes with mucopeptides containing the terminal dipeptide D-alanyl-D-alaninee) ; this interaction within the bacterial cell inhibits crosslinking of the...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1965
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)97178-4